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Laboratory:

Dr. Fereshteh Motamedi

  • Synaptic plasticity laboratory
  • Behavior laboratory
  • Patch clamp recording laboratory

Dr. Abolhasan Ahmadiani

  • Laboratory of Molecular Neurobiology

Dr.Gila Behzadi

  • Neurobiology Laboratory

Dr. Narges Hosseinmardi

  • Field potential recording Lab

Dr. Mahyar Janahmadi

  • Sharp intracellular recordings and Whole cell patch–clamp Laboratory

Dr. Masoumeh Jorjani

  • Laboratory of Neuroendocrinology

 Dr. Leila Dargahi

  • Laboratory of Molecular Neuropharmacology

 Dr. Mir Shahram Safari

  • Lab for Dynamics of Cortical Circuits

 

Dr. Homa Manaheji

  • Laboratory of Pain

Dr. Abbas Haghparast

  • Single unit electrophysiology Laboratory

Dr.Nima Naderi

Dr. jamal Shams

  • Behavior Laboratory

Dr. Afsaneh Eliassi

  • Single channel recording (ion channel reconstitution into artificial bilayers)

Dr.Nader Maghsoudi

  • Cellular and Molecular Biology Laboratory

Dr. Masoumeh Sabetkasaei

  • Pain Laboratory
  • Laboratory of kindling

 Dr. Fariba Khodagholi

  • Neurochemistry laboratory

 

Dr. Fereshteh Motamedi    

PhD Physiology

Professor of Physiology Department and director of Neuroscience Research Center 

1-Synaptic plasticity laboratory

In this laboratory, long – term and short – term synaptic plasticity are being investigated. In this regard, field potential recordings are performed in dentate gyrus and CA1 region of hippocampus in freely moving or unconscious rats by induction of long- term potentiation (LTP), which is believed to be a useful model of the neural mechanism of learning and memory, as well as paired pulse stimulation. The effect of stimulation and reversible inactivation of various brain nuclei on rat’s hippocampal synapses is investigated.

2-Behavior laboratory

In this laboratory, behavioral techniques including learning and memory using T maze, passive avoidance Morris Water Maze, pain mechanisms using tail flick, hot plate, formalin test, and stress induction tests in some physiological and pathological states such as addiction are being investigated in mice and rats.

3- Patch clamp recording laboratory

In collaboration with Dr Nima Naderi in this laboratory the whole-cell patch clamp recordings are performed on neurons in the brain slice preparations of hypothalamus and hippocampus regions. Our work focuses on specific alterations to particular synaptic compounds like cannabinoids as possible mechanisms mediating the modulation of neurotransmission. 
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Dr. Abolhasan Ahmadiani

Professor, Neuroscience Research Center 

Laboratory of Molecular Neurobiology

 

Best values: respect, integrity, creativity, agility and teamwork

 

By employing multidisciplinary approaches which include molecular, cellular, histological and behavioral studies, this laboratory is aimed to advance the understanding of the molecular, cellular and system level functions of central nervous system in health and pathological states. The focus is mainly on neurological and neuropsychiatric disorders, sharing a striking number of common pathological features including neuroinflammation and neurodegeneration.

This laboratory provides research direction toward preventive and/or regenerative medicine with the long-term goal of transition from basic science to applied.

 

Molecular biology techniques:

 

PCR, RT-PCR and qRT-PCR (real-time); Gene Cloning; Lentiviral-mediated gene overexpression/ gene silencing; Immunoblotting techniques including Dot blot, Western Blot, Immunocytochemsistry and immunohistochemistry.

Animal cell and tissue culture techniques:

Primary neuronal cell culture, organotypic culture and/or appropriate cell lines including PC12 (rat pheochromocytoma), BV-2 (mouse, C57BL/6, brain, microglial cells) and C6 (rat astrocytoma) are used for ex vivo central nervous system disease modeling, cell signaling studies and drug screening assays.

 

Behavioral techniques:

 

Experimental animals most commonly used in this lab are rats.

A range of different behavioral tests including Morris water maze, Y maze, Passive avoidance test, Novel object recognition test, Novel place recognition test and object-in-place recognition test are used to measure learning, memory, and cognitive performance in experimental animals.

The open field test is used as a common measure of exploratory behavior and general activity in animals, and Rotarod performance test, Beam test, Pole test, and Cylinder test are used for the assessment of motor coordination attributed to the function of dopaminergic nigrostriatal pathway.

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Dr.Gila Behzadi

PhDNeuroscience

Professor of Physiology Department and Neuroscience Research Center 

Neurobiology Laboratory

The neurobiology lab is based on studying the sensorimotor system also the pathological models of Parkinson’s disease ataxia, insomnia, pain and structural alteration of CNS during development in hypothyroidism pups.

The experimental field consists studying:

  • The neuronal pathways
  • Neurotransmitters
  •  Metabolic enzymes
  •  Discovery of the structural changes together with nuclear proteins expression of neurons in relation to neuronal function 

...................................................................................................................................

Dr.Narges Hosseinmardi

Assistant Prof.

Department of Physiology and Neuroscience research center,

Medical School, Shahid Beheshti University of Medical Sciences.

Field potential recording Lab 

  Field potential is the electrical potential- produced by cells that was recorded by extracellular microelectrodes. It represents the integration of synaptic current within a local volume surrounding a single electrode tip. As an action potential is fired in a neuron, channels open on its membrane and the electric current of the ions that flow through them and in the resistive extracellular volume around the neuron generates local changes in extracellular voltage that can be sensed with a recording electrode. Usually, a field potential is generated by the simultaneous activation of many neurons by synaptic transmission because the extracellular signal from a single neuron is extremely small. However, in some areas of the brain, such as the hippocampus, neurons are arranged in such a way that they all receive synaptic inputs in the same area. Because these neurons are in the same orientation, the extracellular signals from synaptic excitation don't cancel out, but rather add up to give a signal that can easily be recorded with a field electrode. This extracellular signal recorded from a population of neurons is the field potential. 

   Commonly, synaptic plasticity events such as long-term potentiation (LTP), long-term depression (LTD), and Depotentiation are investigated by using a stimulation electrode and a single, monopolar field recording electrode. We focus our study of synaptic plasticity to the synaptic response of CA1 pyramidal neurons to Schaffer collateral stimulation in the hippocampus and use this technique to investigate the mechanisms involved in learning and memory, addiction and epilepsy.
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 Dr. Mahyar Janahmadi

PhD Physiology

Professor of Physiology Department and Neuroscience Research Center 

Sharp intracellular recordings and Whole cell patch–clamp

Laboratory

Mahyar Janahmadi and her research group are interested in neurobiology of diseases and neuroprotection in neurodegenerative diseases, such as cerebellar ataxia, Alzheimer and also epilepsy. Our brains accomplish their jobs by generating coordinated patterns of electrical signals in groups

of nerve cells. Research in this laboratory focuses on the cellular and molecular properties of this coordinated electrical behaviour. Circuits, synaptic connections and intrinsic electrical properties particular to individual nerve cells combine to allow neurons to generate the specific firing patterns that ultimately result in behaviors. Research work here concentrates on the electrophysiological characteristics of the cerebellar Purkinje neurons and hippocampal pyramidal cells in physiological and physiopathological conditions, regions of the brain involved in coordinating movements and learning and memory. In summary, much of the current work focuses on three goals: First, the intrinsic electrophysiological changes that occur in neurodegenerative animal models of diseases and the underlying cellular mechanisms. Particular interest is given to the role of ion channels and signaling cascades. Second, the effects of different toxicants, including the heavy metal Pb2+, Al3+ and of organophosphorus compounds, including nerve agents and insecticides, on neuronal functions in vitro and on the electrophysiological properties of ion channels. Third, the electrophysiological effects of herbal medicine on an experimental model of epilepsy.

Laboratory Techniques

Electrophysiological recording including whole cell patch clamp and sharp intracellular recordings (Single and two electrode current and voltage clamp techniques) and intracellular staining from neurons (in slices) and snail neurons.  
............................................................................................................................

Dr. Masoumeh Jorjani

 Academic Appointments: Professor, Pharmacology, Neuroscience

Administrative Appointment: Dean, Medical School; Director, Neurobiology Research Center,

msjorjani@sbmu.ac.ir         ,            Dean.md@sbmu.ac.ir

Address:Chamran Exp. Way, Velenjak, Shahid Beheshti. Univ. Med. Sci, School of Medicine, Neurobiology Research Center
Phone: 98-21-22439950
Fax: 98-21-22439942
Lab: 98-21-22429765 (Ext. 120)

Personal History:

I graduated from School of Pharmacy at the Tehran University of Medical Sciences in April 1988 with Pharm D. I was then employed 2 years as a lecturer in Department of Pharmacology, School of Medicine at the Shahid Beheshti University of Medical Sciences in Tehran where I received my PhD in November 1995. I worked there as an Assistant Professor and was promoted to Associate Professor in December 1999. From 1999- 2005, I was vice –chancellor for research at the Shahid Beheshti University of Medical Sciences. I received my professorship in April 2004. From Dec. 2007 – Jul. 2008, I received an appointment as Visiting Prof. in the Department of Physiology, Baltimore School of Medicine at the University of Maryland, USA.

Since November 2007, I am General Secretariat for Iranian society of Physiology and Pharmacology and Editor in-chief for “Pejouhandeh” research journal for ten years. I am also a member of the International Association for the study of pain, International Brain Research Organization, Society for Iranian Pharmacist and Iranian Society of Neuroscience. I am currently Dean of Medical School and Director of Neurobiology Research Center at Shahid Beheshti Univ. Med. Sci, Tehran, IRAN.

Research Interests:

We are interested on sex differences in pain perception and pain signaling pathways in the CNS. We investigated the influence of steroid hormones on the pain mechanisms and neurotransmitter release in different brain regions. In recent years, we have focused on mechanisms underlying central neuropathic pain induced by spinal cord injuries and pain modulation by steroids. We found that Estradiol as a multi-active agent protects the neurons and prevents the progress of neurodegeneration. We think that local steroid production is an endogenous mechanism for repair of neural tissue and improvement of somatosensory functions including central pain during CNS damage.

Lab Techniques:

We use a broad range of techniques, from behavioral analysis to the molecular level. Behavioral tests include tests of acute and chronic pain models and motor activity. We also measure the gene expression and detect the interested proteins in the spinal or brain tissues by RT PCR, Immunocytochemistry, Western blot, ELIZA and other molecular techniques. We also study the neurotransmitter release in different regions of CNS by using brain and spinal microdialysis in anesthetized or freely moving rodents. We use HPLC to detect the concentration of molecules of interest in dialysate and other biological samples.

Laboratory Personnel:

  •  Abbas Alimoradian, Post Doc
  •  Reza Ahadi, PhD student
  •  Asiyeh Naderi, Research associate
  •  Ehsanollah Sadeghi, PhD student
  •  Kalantari, Graduate Medical Student
  •  Karkhaneh, Graduate Medical Student
  •  Fatemeh Abbaszadeh - Research Assistant

PublicationsSearch all of my publication in PubMed  Click Here
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 Dr. Masoumeh Sabetkasaei

PhD Pharmacology

Professor of Pharmacology Department and Neuroscience Research Center 

1-Pain Laboratory

Different tasks are performed in this laboratory utilizing different instruments in order to investigate the analgesic and hyperalgesic actions of certain drugs in rodents.

For measuring pain threshold we use hot plate and tail flick apparatus. Also Von Frey test is used for evaluation of hyperalgesia. In addition, we use formalin test in order to test chronic and acute pain. In this laboratory we are expert in intrathecal and intracerebral injection of drugs.

2-Laboratory of kindling

Kindling is a conventional model for inducing temporal lobe epilepsy in which the seizure is similar to focal seizures. In this model, seizures are induced by electrical stimulation of certain area of the brain which secondarily results in generalized seizure. This model provides a valuable tool for studying the effect of intracerebral infusion of antiepileptic drugs. In this laboratory kindling is used for investigation of the following goals:

  • Mechanism of action of antiepileptic drugs
  •  Chronic use of antiepileptic drugs
  •  Antiepileptic drug resistance 

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 Dr. Afsaneh Eliassi

PhD Physiology

Professor in Physiology Department and Neuroscience Research Center, 

Single channel recording (ion channel reconstitution into artificial bilayers)

The defining action of an ion channel protein is to allow an electrical current of ions to flow passively across a cell membrane. Thus, among the variety of approaches, perhaps the most basic measurement of channel function is its unitary current. Single channel currents can be recorded with techniques that involve patch pipettes or artificial bilayers. Ion channel reconstitution involves the incorporation of channel proteins into artificial phospholipid bilayers so permitting the characterization of channel function under voltage clamp conditions. In practice, this term is applied both to procedures which involve true reconstitution (i.e. studies involving purified channel proteins), and to protocols in which intact isolated membrane vesicles, containing the channel protein are incorporated into an artificial membrane.

Ion channel reconstitution provides:

  •  An alternative approach to the measurement of the function of ion channel under volt- age clamp conditions
  •  Experimental strategies which make possible the investigation of channels which are not accessible to conventional electrophysiological approaches (for example the channels of intracellular membrane system)
  •  Methods for assessment of the properties of purified ion channel protein molecules
  • At this time, we are considering the biophysical and electro-pharmacological properties of a potassium channel in:
  • Rough endoplasmic reticulum membrane in intact rat hepatocytes
  • Rough endoplasmic reticulum membrane in diabetic rat hepatocytes
  • Brain mitochondrial inner membranes in intact rats

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Dr.Nader Maghsoudi

PhD Cellular and Molecular Biology

Professor, Neuroscience Research Center 

Cellular and Molecular Biology Laboratory

These laboratories in neuroscience research center are established in order to study several neurodegenerative diseases like Parkinson, Alzheimer . . . at cellular and molecular level. Facilities in these labs permit several techniques like PCR, Western blotting, cloning, DNA&protein purification to be performed.

The main projects in these laboratories are as follows:

  •  To understand pathogenesis of CVB3,a pathogen of infants CNS and also causative of DCM. In this research a model for studying the onset of disease has been developed using HelaTeton system and approaches in order to control the infection. by controlling apoptosis is being investigated. Some results have been published.
  • Other project is directed towards differentiating stem cells to dopaminergic neurons and expressing anti stress genes in these cells, cells are implanted in mouse Parkinsonian model’s brain.
  • Multiple Sclerosis is a neurodegenerative disease where myelin sheaths in nervous system are destroyed. One project is about development of Differentiated stem cells which can act as supportive cells in order to terminate the disease. Progress is being done.
  •  In other project by employing genomic & proteomic tools, effect of stress on hippocampus tissue of rats is being studied.
  •  These laboratories also give services to several other projects carried in physiology, pharmacology and otherdepartments of the university 

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 Dr. Homa Manaheji

PhD Physiology

Associate Professor of Physiology Department and Neuroscience Research Center 

Laboratory of Pain

The aims of this lab are as fellows

  •  Induction of different animal pain models similar to human, such as neuropathic, inflammation and acute pain
  • Electrophysiological study of pain pathways in peripheral and central nervous system using field potential recording from peripheral nerves and single unit recording from spinal cord cells.
  •  Using different drugs and herbal medicine to understand pain mechanism 

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Dr. Abbas Haghparast

PhD Physiology

Professor, Neuroscience Research Center 

Single unit electrophysiology Laboratory

The work in this lab is devoted to extracellular single-unit recording of neural activity in different brain regions. Particular attention is given to brain regions implicated in pain modulatory systems and addiction. We place anesthetized rats in

a stereotaxic instrument, and approach to the predefined position within the nucleus of interest to record neural activity associated to adjacent neuronal cells. Only single cells having a consistent spike amplitude and waveform during the experimental procedure are studied. Spikes reflecting neural activity are isolated from background activity. An increase or decrease of firing rate over two-fold of the standard deviation of the baseline activity for at least 3 consecutive bins (i.e., 3 min) is considered as an excitatory or inhibitory response, respectively. Event-related firing rate and pattern are analyzed. After the experiment, the rat is perfused transcardially, brain is removed and fixed followed by preparing coronal sections and staining for histological verification of the site of microinjection and/or recording. 

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 Dr.Nima Naderi

Pharm D, PhD Toxicology

Associate Professor, Neuroscience Research Center 

Dr. Naderi’s research activities focus on the effects of cannabinoid compounds on a variety of different psycho- logic and pathophysiologic conditions such as anxiety, depression, seizure and pain and on the synaptic pharmacology of brain regions such as the supraoptic nucleus of the hypothalamus and dentate gyrus of hippocampus. Techniques used:

  •  Whole cell current and voltage clamp in brain slices (in collaboration with Dr. Fereshteh Motamedi)
  • Elevated plus maze test and light-dark test for evaluating anxiety-related behavior of rats and mice
  •  Pentylenetetrazole and maximal electroshock models of acute seizure in mice, chemical and electrical models of kindling in rats
  •  Tail flick, hot plate and formalin tests for evaluating the pain related behaviors of animals

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 Dr. jamal Shams

Associate Professor of Psychiatry Department and Neuroscience Research Center

Behavior Laboratory

In this laboratory, psychiatric disorders such as addiction, anxiety (posttraumatic stress disorder) and tardive dyskinesia secondary to prescription of anti psychotic drugs and sexual dysfunctions such as premature ejaculation are being studied by using the behavioral techniques in the animal models.

In the last studies the effects of some herbal medicines have been used for treatment of post traumatic stress disorder, tardive dyskinesia substance dependency and attenuating pain severity in animal models.

The instruments which are used in this laboratory are: Conditioning place preference (CPP), Plus maze, Ethovision (for recording and analyzing behavior), activity monitoring, tail flick and hot plate for studying pain.

...............................................................................................................................

 Dr Leila Dargahi

Assistant Professor, Neuroscience Research Center

 

Laboratory of Molecular Neuropharmacology

 

Best values: respect, integrity, creativity, agility and teamwork

 

By employing multidisciplinary approaches which include molecular, cellular, histological and behavioral studies, this laboratory is aimed to advance the understanding of the molecular, cellular and system level functions of central nervous system in health and pathological states. The focus is mainly on neurological and neuropsychiatric disorders, sharing a striking number of common pathological features including neuroinflammation and neurodegeneration.

This laboratory provides research direction toward preventive and/or regenerative medicine with the long-term goal of transition from basic science to applied.

 

Molecular biology techniques:

 

PCR, RT-PCR and qRT-PCR (real-time); Gene Cloning; Lentiviral-mediated gene overexpression/ gene silencing; Immunoblotting techniques including Dot blot, Western Blot, Immunocytochemsistry and immunohistochemistry.

Animal cell and tissue culture techniques:

Primary neuronal cell culture, organotypic culture and/or appropriate cell lines including PC12 (rat pheochromocytoma), BV-2 (mouse, C57BL/6, brain, microglial cells) and C6 (rat astrocytoma) are used for ex vivo central nervous system disease modeling, cell signaling studies and drug screening assays.

 

Behavioral techniques:

 

Experimental animals most commonly used in this lab are rats.

A range of different behavioral tests including Morris water maze, Y maze, Passive avoidance test, Novel object recognition test, Novel place recognition test and object-in-place recognition test are used to measure learning, memory, and cognitive performance in experimental animals.

The open field test is used as a common measure of exploratory behavior and general activity in animals, and Rotarod performance test, Beam test, Pole test, and Cylinder test are used for the assessment of motor coordination attributed to the function of dopaminergic nigrostriatal pathway.

...................................................................................................................

Dr. Fariba Khodagholi

Associate Professor, Neuroscience Research Center 

Neurochemistry laboratory:

The Neurochemistry laboratory of the Neuroscience Research Centre of Shahid Beheshti Medical University is devoted to the study of neuroscience and brain researches in health and disease. By integrating methodologies we aim to advance the understanding the mechanism and treatment of complex brain disorders.

The main objective of our neurochemistry laboratory in neuroscience research center is cellular, molecular and biochemical studies of biomolecules, neurochemicals and pharmaceutical drugs on nervous system structure and function in vivo and/or in vitro.

Our neurochemistry laboratory uses Neural Cell Culture, Enzymology, Immunohistology, and western blot techniques. Additional research directions are the studies of interactions between biochemical and behavioral and/or electrophysiological data in case of neurodegenerative diseases, stroke, traumatic brain injury, addiction, and epilepsy. In this way we can assess the neurochemical aspects of the neurological disorders and also effects of the treatment on them.

............................................................................................................................................................

Dr. Mir Shahram Safari

PhD Physiology
Lab for Dynamics of Cortical Circuits 

Importance of top-down (corticocortical) and bottom-up (subcortical) pathways in regulating visual responsiveness are crucial, how these two pathways working independently and how interact during coincident engagement in vivo to optimize visual perception while maintaining network stability is unknown. Sensory-evoked responses in V1 neurons are highly dependent on subcortical neuromodulation pathways that regulate brain state. With cell-type-specific resolution using optogenetics and optopatcher in vivo, we are addressing how corticocortical and subcortical pathways (such as cholinergic and serotonergic systems) interact to regulate responsiveness of layer II/III neurons of V1. Our studies provide insight into the rules and conditions governing activity propagation in connected networks of brain.

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t Iran, Tehran
Velenjak St. , Shahid Chamran Highway
Tel : +98 21 22 43 99 82
Fax : +98 21 22 43 97 84
Email:     " Intl_office@sbmu.ac.ir "

 

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